Projects

Ultrasound technique for the study of blood coagulation system:

We are using ultrasound technique for the study of coagulation and fibrinolysis phenomena in our original experimental set-up. In particular this set-up allows us to analyze the physiological effect of the existing pharmaceutical drugs.

Three demonstrations of the blood coagulation are shown below in the movies:

	Fibre-like fibrin structure floating in the blood plasma flow in vitro The video shows a scenario of the development of blood plasma coagulation in the experimental in vitro system consisting of silicone tubes. Fibre-like fibrin structures floating in the stream are clearly visible. An audio track of the video is a Doppler frequency shift of a signal reflected by a stream. The signal amplitude is correlated with fibrin clots quantity and size into experimental system. See more details in the article [PhilosTrans, 2008].
	Macroscopic thrombi formation in the blood plasma under intensive flow conditions in vitro Thrombus formation processes can develop very rapidly under intensive blood flow. Over a time of about ten minutes the macroscopic thrombus completely blocks the lumen of the in vitro model of the vessel. In this process, blood coagulation develops consistently: the formation of primary microclots, the formation of more large aggregates, the formation of а macrothrombus. See more details in the article [Biophysics, 2018].
	Hydrodynamic activation of blood coagulation in the stenotic vessel in vitro Here is a typical scenario of the rapid formation of large thrombs that occur in the flow for a few minutes.

This research direction has been supported by grants of the Russian Foundation for Basic Research:
- N 14-04-01193 (2014-2016), «Исследование влияния условий формирования тромба на его резистентность к действию фибринолитических агентов»;
- N 07-04-01523-a (2007-2009), «Исследование спонтанного тромбообразования в интенсивных потоках»;
- N 04-04-49423-a (2004-2006), «Акустические эффекты при свертывании крови в интенсивных потоках».

Personalized maps of potential thrombogenic danger:

Another research direction is the development of unique software and hardware complex, allowing to build personalized maps of potential thrombogenic danger for different areas of cardiovascular system of individual patients, and also to produce video material, which illustrates possible activation scenarios of intravascular coagulation processes. Creation of maps of potential thrombogenic danger will open new possibilities not only for doctors but also for patients to evaluate a priori, more fully and deeply the structure of the actual risks of the development of extremely dangerous pathologies associated with cardiac and circulation disorders. Illustrative video materials are available in the section Videos and on the website www.in-silico.ru.

Spatial-temporal distribution of activated platelets in the model of a large artery The elements of the developed mathematical model, which takes into account both biochemical and hydrodynamic features of blood, can be found in our articles [Scientific Reports, 2016; PLoS ONE, 2015].

Our website in-silico.ru serves to demonstrate the possibilities of mathematical modeling of systemic hemodynamics, taking into account the phenomena of intravascular thrombus formation. The project is designed to expand the possibilities of personalized medicine through the use of modern methods of computer modeling (within the framework of the in silico approach). A web service is being developed to predict the personalized risks of thrombotic complications and areas of increased thrombogenic risk.

This research direction has been broadly funded by various grants:
- Russian Science Foundation (RSF) N 19-11-00260 (2019-2021), «Investigation of thrombogenesis activation in intensive blood flow»;
- Russian Science Foundation (RSF) N 14-14-00990 (2014-2016), «Mathematical modeling of blood coagulation phenomena in human cardiovascular system»;
- International Science and Technology Center (ISTC) N 3744 (2007-2010), «Computational Modeling of Blood Hydrodynamics and Aggregation Phenomena in the Human Cardiovascular System»;
- Russian Foundation for Basic Research (RFBR): N 99-04-48759-a (1999-2001), «Роль нестабильностей в динамике крови»; N 99-01-01145-a (1999-2001), «Математическое моделирование тромбообразования в сосудах сложной геометрии с использованием моделей неньютоновских жидкостей»; N 96-01-01306 (1996-1997), «Численное исследование неравновесных диссипативных структур в моделях тромбообразования».

Currently, novel research approach known as in silico is actively developed by scientists and clinicians. The demonstration video "In silico medicine will be the future", prepared by the international non-profit organization Virtual Physiological Human Institute, gives a general idea of the approach.